| 质粒名称: | pD603 H77 E1E2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 86983 |
| 存储实验室: | Joe Grove |
|
载体骨架基本信息 |
|
| 载体名称: | pD603 |
| 载体出品公司: | DNA 2.0 |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | HCV E1E2,AAB67036.1 |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pD603 H77 E1E2 was a gift from Joe Grove (Addgene plasmid # 86983 ; http://n2t.net/addgene:86983 ; RRID:Addgene_86983) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pD603 H77 E1E2 6713 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Mammalian expression vector encoding codon optimised Heptitis C
virus H77 glycoproteins E1E2.
ACCESSION .
VERSION .
KEYWORDS pD603 H77 E1E2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6713)
TITLE Grove lab plasmids
REFERENCE 2 (bases 1 to 6713)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6713
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 23..611
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 512..531
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 765..782
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 880..897
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 881..897
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 1353..1369
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
enhancer 1396..1775
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1776..1979
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 1930..1950
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 2024..2043
/label=T7
/note="T7 promoter, forward primer"
promoter 2024..2042
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 2073..2082
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
primer_bind complement(3834..3851)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 3840..3951
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(4069..4089)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4074..4403
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4254..4389
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4316..4335
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4470..5264
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4524..4543)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5134..5153
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 5438..5559
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5475..5494)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5529..5548
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
CDS complement(5612..6472)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6235..6254
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6473..6564)
/gene="bla"
/label=AmpR promoter
primer_bind 6606..6625
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 6631..6650
/label=pENTR-R
/note="pENTR vectors, reverse primer"
ORIGIN
1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
181 agccgtagtt agcccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
721 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
781 aggaagcgga aggcgagagt agggaactgc caggcatcaa actaagcaga aggcccctga
841 cggatggcct ttttgcgttt ctacaaactc tttctgtgtt gtaaaacgac ggccagtctt
901 aagctcgggc cccctgggcg gttctgataa cgagtaatcg ttaatccgca aataacgtaa
961 aaacccgctt cggcgggttt ttttatgggg ggagtttagg gaaagagcat ttgtcagaat
1021 atttaagggc gcctgtcact ttgcttgata tatgagaatt atttaacctt ataaatgaga
1081 aaaaagcaac gcactttaaa taagatacgt tgctttttcg attgatgaac acctataatt
1141 aaactattca tctattattt atgatttttt gtatatacaa tatttctagt ttgttaaaga
1201 gaattaagaa aataaatctc gaaaataata aagggaaaat cagtttttga tatcaaaatt
1261 atacatgtca acgataatac aaaatataat acaaactata agatgttatc agtatttatt
1321 atcatttaga ataaattttg tgtcgccctt aattgtgagc ggataacaat tacgagcttc
1381 atgcacagtg gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
1441 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
1501 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
1561 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
1621 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
1681 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
1741 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
1801 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
1861 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
1921 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
1981 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
2041 ggggagagac cacacccaag ctgtctagag ccgccaccat gggctgcagc ttcagcatct
2101 tcctgctggc cctgctgagc tgtctgacag tgcctgcctc tgcctaccaa gtgcggaata
2161 gcagcggcct gtaccacgtg accaacgact gccccaatag cagcatcgtg tacgaagccg
2221 ccgatgccat cctgcacaca cctggatgtg tgccctgtgt cagagagggc aacgccagca
2281 gatgttgggt tgccgtgaca cctaccgtgg ccaccagaga tggaaagctg ccaaccacac
2341 agctgcggag acacatcgat ctgctcgtgg gaagcgccac actgtgtagc gctctgtatg
2401 tgggcgatct gtgcggctcc gtgtttctcg tgggccagct gttcacattc agccccagaa
2461 ggcactggac cacacaggac tgcaactgca gcatctaccc cggccacatc accggacata
2521 gaatggcctg ggacatgatg atgaattgga gccccacagc cgctctggtg gttgctcagc
2581 tgctgagaat ccctcaggcc atcatggaca tgatcgctgg cgctcattgg ggagtgctgg
2641 ccggaatcgc ctacttcagc atggtcggaa actgggccaa agtgctggtg gtcctgctgc
2701 tgtttgctgg cgtggacgcc gaaacacatg tgacaggcgg aagcgccgga agaacaacag
2761 ctggacttgt gggcctgctg acacctggcg ccaagcagaa tatccagctg atcaacacca
2821 acggcagctg gcacatcaac agcacagccc tgaactgcaa cgagagcctg aataccggat
2881 ggctggccgg cctgttctac cagcacaagt ttaacagcag cggctgcccc gagagactgg
2941 cctcttgtag aaggctgacc gacttcgccc aaggctgggg ccctatctct tacgccaatg
3001 gctctggcct ggacgagagg ccttactgct ggcactatcc tccaagacct tgcggcatcg
3061 tgcctgccaa gtctgtgtgt ggacccgtgt actgcttcac cccatctcca gtggtcgtgg
3121 gcaccaccga tagatctggc gccccaacat atagctgggg agccaacgac accgacgtgt
3181 tcgtgctgaa caataccaga cctcctctcg gcaattggtt cggctgtacc tggatgaaca
3241 gcaccggctt caccaaagtg tgcggagccc ctccttgtgt gattggcggc gtgggaaaca
3301 acaccctgct gtgccctacc gactgcttca gaaagcaccc tgaggccacc tacagcagat
3361 gcggatctgg cccttggatc acccctagat gcatggtgga ctacccctac cggctgtggc
3421 actacccttg caccatcaac tacaccatct tcaaagtgcg gatgtacgtc ggcggagtgg
3481 aacacagact ggaagccgcc tgcaattgga ccagaggcga gagatgcgac ctggaagatc
3541 gggacagaag cgagctgtct cctctgctgc tgagcaccac acagtggcag gtcctgcctt
3601 gctccttcac cacactgcca gctctgagca ccggcctgat tcatctgcac cagaacatcg
3661 tggacgtgca gtacctgtac ggcgtgggca gctctattgc cagctgggcc atcaagtggg
3721 agtacgtggt gctcctgttc ctgctcctgg ccgatgctag agtgtgcagc tgcctgtgga
3781 tgatgctgct gatctctcag gccgaagcct gaggttgagg tctctaaaat cagcctcgac
3841 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
3901 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cacaacactc
3961 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
4021 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
4081 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
4141 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
4201 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
4261 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
4321 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
4381 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
4441 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
4501 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
4561 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
4621 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
4681 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
4741 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
4801 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
4861 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
4921 gtcttgtcga tcaggatgat ctggacgagg aacatcaggg gctcgcgcca gccgaactgt
4981 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
5041 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
5101 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgagg
5161 aacttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
5221 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
5281 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
5341 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
5401 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
5461 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
5521 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
5581 ctctagctag agcttggcgt aatcatggtc attaccaatg cttaatcagt gaggcaccta
5641 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
5701 ctacgatacg ggagggctta ccatctggcc ccagcgctgc gatgataccg cgagaaccac
5761 gctcaccggc tccggattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
5821 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
5881 taagtagttc gccagttaat agtttgcgca acgttgttgc catcgctaca ggcatcgtgg
5941 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
6001 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
6061 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
6121 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
6181 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
6241 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
6301 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
6361 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
6421 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atattcttcc
6481 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
6541 aatgtattta gaaaaataaa caaatagggg tcagtgttac aaccaattaa ccaattctga
6601 acattatcgc gagcccattt atacctgaat atggctcata acaccccttg ctcatgacca
6661 aaatccctta acgtgagtta cgcgcgcgtc gttccactga gcgtcagacc ccg
//
